Cellular Imaging Instrumentation Accessories
INTACT GENOMICS INC ig Recombinase Polymerase Amplification (RPA) Kit v1, 500 Reactions
The ig RPA Kit version 1 provides the necessary reagents and enzymes to amplify user-provided DNA template using isothermal Recombinase Polymerase Amplification. It requires no thermocycling with equal or better sensitivity than PCR. RPA is a molecular biology technique that amplifies DNA at a constant temperature using a recombinase, primers, a single-stranded DNA binding protein (SSB), and a strand displacing DNA polymerase. T4 UvsX Recombinase is used with UvsY and primers to cause strand exchange of double-stranded DNA. Then, a single-stranded binding protein, T4 gp32, stabilizes the displaced strand. Finally, Bsu or Sau DNA polymerase extends the DNA from the primers and creates a new complete copy of the template. Iterative amplification ensues as with PCR. RPA is a highly selective and sensitive isothermal amplification technique. No DNA pretreatment is required. It is compatible with a variety of endpoint detection methods, (e.g. lateral flow, real-time fluorescence).
Caron Products And Services LED Tissue/Seedling, 1-Door, 22 cu. ft., 300 mole, 4 Tier
LED Plant Growth Chamber. 22 ft3 with 300 mole/m2/s of gBrite LED light, 4 tier, temperature control, touchscreen. 10C to 45C, 115V, 60 Hz.
Cytoskeleton, Inc. SiR-Actin Kit, 50 nmol SiR-Actin and 1 umol verapamil
SiR-actin is based on the F-actin binding natural product jasplakinolide. SiR-actin is fluorogenic, cell permeable and highly specific for F-actin. Sir-actin stains endogenous F-actin without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-actin is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR-actin can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.
Electron Microscopy Sciences Kimball Physics LaB6 Cathode for Leica, Cambridge
LaB6 Cathodes are high performance, resistively heated, thermionic electron source for use in SEM, TEM, electronlithography systems, and x-ray sources. They offer an extended life with thousands of hours in clean vacuum, exceptional thermal, chemical, and electrical stability, high brightness/low energy spread with oriented single crystal made from the best quality/high purity material. Style 90-15 : Standard Microflat
Sartorius Incucyte Neuroactive Kit
The Incucyte Neuroactive Kit contain cryopreserved vials of rat cortical neurons and astrocytes isolated from embryonic stage E18 Sprague-Dawley rats as well as sufficient Incucyte Neuroburst Orange Lentivirus to perform one 96-well experiment.The Incucyte Neuroburst Orange Lentivirus is a lentiviral based live-cell neuronal labeling reagent driven by a synapsin promoter resulting in the long term expression of a genetically encoded orange fluorescent calcium indicator mRuby-based in neuronal cells.
